Assessment of Chattonella subsalsa Cyst and Excysted Cells Isolateted from Sediment of Lipar Zone (Makran Coast) Based on Morphology and LSU-rDNAGen Sequence

This study has been carried out in order to identify the phytoplankton cysts from sediment of Lipar zone (southeast coast of Iran) based on morphology and phylogeny of germinated cell. The sediment samples were collected from three sations by Ekman grab with 225 cm2 collecting area in 2015. Unknown cysts with different morphology were cultured in the petridishei containing the F2 medium and kept in phycolab and Germinator undere a proper condition 12:12 L/D in 25 ºC±1. The germinated cell was similar to the Chattonella subsalsa based on intitial morphological assessment. To confirm the identification, DNA of germinated cell was extracted and PCR and gene sequence of partial LSU-rDNA region were done. Phylogenetic analysis showed that germinated cell with 98% boot strap support was resembeled to the C. subsalsa. The assessment of cyst/theca relationship in the phytoplankton lifecycle can be a useful tool in the identification of the phytoplankton cells.